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Multiphoton Microscopy Sets the Standard for Live-Cell Imaging

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Tunable ultrafast lasers enable 3D imaging and research applications ranging from neuroscience to improved understanding of stem cell transplantation.

Darryl McCoy and Marco Arrigoni, Coherent Inc.

Multiphoton imaging is widely used for live-cell imaging. Many of the applications are in neuroscience, where the overarching trends involve deeper imaging using longer wavelengths. These trends are the leading drivers behind the latest generation of ultrafast lasers based on ytterbium (Yb) fiber. However, there are numerous other applications far outside the well-publicized field of neuroscience, and these are often amply served with traditional titanium:sapphire (Ti:S) ultrafast lasers. Gabriele Bixel and colleagues at the Max Planck Institute for Molecular Biomedicine in Muenster,...Read full article

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    Published: March 2018
    Glossary
    optical parametric oscillator
    A laser-pumped crystal with nonlinear optical properties inside of an optical resonator in which the output generates coherent light whose output can be tuned continuously over a variety of infrared and visible wavelengths. The OPO generates a short wavelength component called the signal and a longer wavelength component called the idler. Conservation of energy dictates that the sum of the photon energy of the signal and that of the idler equals the original pump photon energy.
    FeaturesMicroscopyBiophotonicsLasersTunable LasersDarryl McCoyMarco ArrigoniCoherentoptical parametric oscillator

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