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Neuroscience Driving Continued Growth in Multiphoton Microscopy

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The inherent three-dimensional spatial resolution and live sample compatibility of multiphoton microscopy are a perfect match for high-resolution studies in neuroscience using the latest optogenetic tools.

MARCO ARRIGONI and DARRYL MCCOY COHERENT INC.

More than half of the thousands of deployed multiphoton microscopes are used for neuroscience — in large part because of major funding initiatives across three continents. Some of these initiatives are specifically targeted toward in vivo studies, such as the Brain Research through Advancing Innovative Neurotechnologies (BRAIN) initiative in the U.S. The most advanced of these studies combine multiphoton techniques with optogenetics to enable “all-optical” functional studies of neural networks. Two-photon microscopy is the only method of performing this stimulation and...Read full article

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    Published: June 2016
    Glossary
    three-photon microscopy
    Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It is used in biological and medical research to capture high-resolution images of biological specimens, particularly in thick tissues, without causing significant damage. This technique takes advantage of the nonlinear optical processes that occur when multiple photons interact with a fluorophore to achieve fluorescence imaging. Key concepts behind three-photon microscopy...
    random access multiphoton microscopy
    Also known as RAMP microscopy, random access multiphoton microscopy is a microscopic technique that uses multiple acousto-optic modulators to enable faster and addressable scanning of 3D cellular structures, frequently employed in optogenetics and neuroscience research.
    fiber lasersMicroscopyultrafast lasersLasersBiophotonicsAmericasMulti-Photon Microscopytwo-photon microscopyThree-Photon Microscopyneuroscienceneuronbrain research through advancing innovative neurotechnologiesBRAIN initiativetitanium:sapphireTi:Sytterbium-doped gain materialsstreamlined fixed wavelength lasersytterbium-based ultrafast lasersrandom access multiphoton microscopyFeatures

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