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Nikon Instruments Inc.

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Nikon Instruments Inc. of Melville, N.Y., and 421%%Agilent Technologies of Santa Clara, Calif., have announced an agreement for distribution in the Americas of Agilent’s MLC400 monolithic laser combiner systems. They will be integrated into laser systems designed for Nikon’s Ti-E PFS “Perfect Focus,” an inverted research microscope that performs multiday live-cell imaging experiments. The integration will allow researchers to maximize productivity and minimize system downtime. Agilent’s monolithic laser combiner will achieve high optical efficiency on the...Read full article

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    Published: March 2011
    Glossary
    photobleaching
    Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
    total internal reflection fluorescence
    Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the principle of total internal reflection to selectively illuminate and image a thin section of a specimen near the interface of two media, typically a glass coverslip and the sample medium. TIRF microscopy allows for high-resolution imaging of fluorescently labeled structures near the cell membrane or other interfaces, offering improved signal-to-noise ratios and reduced...
    Agilent TechnologiesBiophotonicsBusinessCaliforniaconfocal microscopyImaginginverted research microscopelive-cell imagingMicroscopyMLC400 monolithic laser combiner systemsNew YorkNikon InstrumentsOpticsphotoactivationphotobleachingRapidScanTi-E PFS Perfect Focustotal internal reflection fluorescenceLasers

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