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Novel Multiphoton Imaging Techniques Reveal Brain’s Secrets

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Two-photon microscopy has surpassed confocal imaging for the study of intact, living tissue. Three-photon imaging is not far behind and is already allowing researchers to image at depths.

FAROOQ AHMED, CONTRIBUTING EDITOR

Nearly two decades ago, Winfried Denk and Karel Svoboda argued in the journal Neuron that “multiphoton imaging is more than a gimmick”1. At the time, confocal microscopy — combined with advancements in fluorescent reporter systems and an increase in desktop computing power —...Read full article

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