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Novel autofocus technique advances high-resolution, time-lapse imaging

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Gary Boas

Development and continued improvement of fluorescent biosensors have provided researchers with access to intracellular protein dynamics in individual living cells — through high-resolution, time-lapse imaging of multiple fields of view, for example. Autofocus often is called on in these studies to compensate for temperature drift, for an uneven substrate across the multiple fields of view and for cell growth over extended periods. Researchers have reported a novel autofocus technique for high-resolution time-lapse imaging. The technique takes advantage of a specially designed bandpass...Read full article

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    Published: September 2008
    autofocusBiophotonicsfluorescent biosensorsintracellular protein dynnamicsMicroscopyResearch & TechnologySensors & Detectors

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