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Photothermal imaging detects nanorod orientation

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Caren B. Les, [email protected]

“Fluorescence vector probes and polarization-sensitive single-molecule spectroscopies have been widely used to explore the conformation dynamics of proteins in biological systems – and an ideal probe should yield high signal with low background noise and have good photostability and biocompatibility with a minimum size to avoid interfering with the system of interest,” explained Wei-Shun Chang, a postdoctoral scientist in the department of chemistry at Rice University. He noted that the conventional probes – organic dyes and quantum dots – suffer...Read full article

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    Published: May 2010
    Glossary
    absorption
    The transfer of energy from an incident electromagnetic energy field with wavelength or frequency to an atomic or molecular medium.
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    nano
    An SI prefix meaning one billionth (10-9). Nano can also be used to indicate the study of atoms, molecules and other structures and particles on the nanometer scale. Nano-optics (also referred to as nanophotonics), for example, is the study of how light and light-matter interactions behave on the nanometer scale. See nanophotonics.
    quantum dots
    A quantum dot is a nanoscale semiconductor structure, typically composed of materials like cadmium selenide or indium arsenide, that exhibits unique quantum mechanical properties. These properties arise from the confinement of electrons within the dot, leading to discrete energy levels, or "quantization" of energy, similar to the behavior of individual atoms or molecules. Quantum dots have a size on the order of a few nanometers and can emit or absorb photons (light) with precise wavelengths,...
    scattering
    Change of the spatial distribution of a beam of radiation when it interacts with a surface or a heterogeneous medium, in which process there is no change of wavelength of the radiation.
    absorptionalignmentBasic ScienceBiophotonicsCaren B. Lesconformational dynamicsdark field scattering microscopydiode lasersDisplaysfluorescencefluorophoresgold nanoparticlesImagingindustrialMetallic nanoparticlesMicroscopynanoNanorodsnanostructuresOpticsorientationorientation sensorsphotothermal imagingPNASpolarization sensitive photothermal imagingprobesproteinsquantum dotsResearch & TechnologyRice UniversityscatteringSensors & DetectorsStephan Linksurface enhanced Raman spectroscopy (SERS)surface plasmonTech PulseTexasWei-Shun ChangLasers

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