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Raman Spectroscopy and Microscopy Enable Life Science Discoveries

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Dr. Marinella G. Sandros, University of North Carolina at Greensboro, and Dr. Fran Adar, Horiba Scientific

The first article in this two-part series looked at Raman spectroscopy applications in tumor surgery, endoscopy and bacterial identification. This second part examines applications in cell sorting, laser trapping, biology probing and more. The field of medical diagnostics will have tremendous potential gains in the near future due to Raman spectroscopy and microscopy. Applications include surface-enhanced Raman spectroscopy (SERS), signal enhancement targeted to specific analytes, coupling atomic force microscopy (AFM) systems and enabling tip-enhanced Raman spectroscopy (TERS) for...Read full article

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    Published: March 2015
    Glossary
    raman spectroscopy
    Raman spectroscopy is a technique used in analytical chemistry and physics to study vibrational, rotational, and other low-frequency modes in a system. Named after the Indian physicist Sir C.V. Raman who discovered the phenomenon in 1928, Raman spectroscopy provides information about molecular vibrations by measuring the inelastic scattering of monochromatic light. Here is a breakdown of the process: Incident light: A monochromatic (single wavelength) light, usually from a laser, is...
    laser trapping
    A technique for confining atoms, molecules or small particles within one or more laser beams. This can be accomplished through the use of a single focused beam or multiple intersecting beams. With a single focused beam, the matter is confined to the laser beam's focal area. In the case of multiple intersecting beams, the matter is confined to the area of intersection because of the combined cooling effect of the beams. Also called optical trapping.
    optical tweezers
    Optical tweezers refer to a scientific instrument that uses the pressure of laser light to trap and manipulate microscopic objects, such as particles or biological cells, in three dimensions. This technique relies on the momentum transfer of photons from the laser beam to the trapped objects, creating a stable trapping potential. Optical tweezers are widely used in physics, biology, and nanotechnology for studying and manipulating tiny structures at the microscale and nanoscale levels. Key...
    BiophotonicsHoribaUniversity of North Carolina at GreensboroDr. Marinella G. SandrosDr. Fran Adar of Horiba ScientificMicroscopyFeaturesRaman spectroscopytumor surgerySERSTERSlaser trappingatomic force microscopycell sortingcoherent anti-Stoke Raman scatteringbacterial identificationoptical tweezersDisease DetectionAmerican Chemical SocietyhypoxanthineNorth Carolina

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