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Single-Molecule Localization Microscopy Approach Improves Precision

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An interferometric, single-molecule localization method for superresolution fluorescence microscopy, developed by scientists at the Institute of Biophysics of the Chinese Academy of Sciences, could significantly improve localization precision compared with conventional centroid fitting methods. The new approach is called Repetitive Optical Selective Exposure (ROSE). It was developed by professors Tao Xu and Wei Ji. The ROSE technique uses six different direction and phase interference fringes to excite fluorescent molecules. The intensity of the molecules is closely related to the phase...Read full article

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