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Seeing More with Less

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Hank Hogan

When it comes to fluorescence microscopy, a team of investigators has shown that you can see more with less. Scientists at the Janelia Farm Research Campus of Howard Hughes Medical Institute (HHMI) in Ashburn, Va., at the National Institute of Child Health and Human Development in Bethesda, Md., at Florida State University in Tallahassee and at NuQuest Research LLC in La Jolla, Calif., imaged fluorescent proteins within cells with ~2- to 25-nm resolution. That is far below the classical resolution limit of half a wavelength, or a few hundred nanometers in the visible. “Resolution is a...Read full article

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    Published: October 2006
    Glossary
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    Featuresfluorescence microscopyMicroscopynanometersoptical microscopy

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