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Silicone Immersion Objectives Answer the Call for Higher Resolution

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High-resolution 3D imaging is now possible over prolonged periods.

ANDREW SAMUELSSON, OLYMPUS CORPORATION OF THE AMERICAS, SCIENTIFIC SOLUTIONS GROUP

Refractive index mismatch, and resulting spherical aberration, has historically plagued researchers interested in long-term, live-cell imaging at high resolution. The use of silicone oil objectives helps mitigate this mismatch as the refractive index of live cells (n = 1.38) is much closer to that of silicone oil (n = 1.4) than that of either traditional immersion oil (n = 1.52) or water (n = 1.33). When used as an immersion medium, silicone oil greatly reduces spherical aberration, which is the inability of light rays to come to a single focal point in Z. As a result, brighter and...Read full article

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