Ruisheng Lin, Alex Clowsley, Isuru Jayasinghe and Christian Soeller, Biomedical Physics, University of ExeterEMCCD-based cameras with high quantum efficiencies and low readout noise characteristics traditionally have been the preferred technology for SMLM superresolution imaging. However, with tailored localization algorithms, sCMOS cameras have become a notable alternative for superresolution microscopy. The ability of optical superresolution microscopy to circumvent the so-called “diffraction limit” has revolutionized the use of fluorescence microscopy to study subcellular and molecular-scale biological processes. 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