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Technique produces wide-field optical images with nanometer resolution

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Images resolve individual vesicles

Hank Hogan

Researchers would like to obtain wide-field images of biological processes with nanometer resolution without destroying the sample. Although methods that produce this level of resolution do exist, none meet all these conditions. However, postdoctoral researcher Alexey Sharonov and physical chemistry professor Robin M. Hochstrasser of the University of Pennsylvania in Philadelphia have now devised a way to perform rapid and relatively simple wide-field imaging at a nanometer scale. Their technique combines concepts used in other approaches: photobleaching, point-spread function measurements...Read full article

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    Published: March 2007
    Basic ScienceBiophotonicsenergyfluorescent probesMicroscopynanometer resolutionResearch & Technologywide-field images of biological processes

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