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Ultrafast Lasers Excite Advancements in Multiphoton Imaging

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The next generation of ultrafast lasers is enhancing multiphoton microscopy by penetrating deeper into biological tissue and generating higher-resolution images.

CHRISTOPHER LEBURN, CHROMACITY LTD., AND CHRISTIAN WILMS, SCIENTIFICA LTD.

Multiphoton microscopy can image deeper into tissue than traditional confocal microscopy, making it ideal for a range of life science studies. The technique is crucial for brain-related research because it allows imaging of large numbers of neurons or individual synapses and can also be performed in live animals. Today, most multiphoton imaging research employs two-photon techniques in which fluorescent dyes that emit light upon excitation are used. Commonly, a fluorescence molecule is promoted to an excited state by absorbing a single excitation photon. But two photons of half the...Read full article

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    Published: September 2021
    FeaturesLasersImagingMicroscopyfiber lasersultrafast lasersTunable LasersBiophotonics

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