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What's New with PCR

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By Caren B. Les, News Editor
[email protected]

Polymerase chain reaction (PCR), the workhorse of biological analysis, is used in areas ranging from criminal investigations and disease diagnosis to biological research. Sometimes referred to as “molecular photocopying,” it is the test, for example, that determines whether a strand of hair found at a crime scene matches that of a certain suspect. The technique, which incorporates optical components and fluorescence, uses a heat-resistant DNA polymerase from the thermophilic bacterium Thermus aquaticus to accurately replicate manifold small segments of DNA for genetic and molecular...Read full article

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    Published: September 2009
    Glossary
    photonics
    The technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. The science includes light emission, transmission, deflection, amplification and detection by optical components and instruments, lasers and other light sources, fiber optics, electro-optical instrumentation, related hardware and electronics, and sophisticated systems. The range of applications of photonics extends from energy generation to detection to communications and...
    polymerase chain reaction
    The polymerase chain reaction (PCR) is a widely used molecular biology technique used to amplify a specific segment of DNA (deoxyribonucleic acid) through a series of temperature-controlled cycles. PCR enables the exponential amplification of a targeted DNA sequence, making it possible to generate millions to billions of copies of the DNA segment of interest from a small starting amount. The PCR process typically involves three main steps: Denaturation: The double-stranded DNA template is...
    assaysBasic ScienceBiophotonicsBioTroveBrenanCaren B. LesColin Brenandiagnostic testDNAfluorescence imagingfluorescent dyegenetic analysisH1N1industrialinfluenzaLaetitia NinovaLESLife TechnologiesLightCyclermicroarrayMicroscopymolecular analysisNaohiro NodaNational Institutes of HealthNIHNinovaNodanucleic acidsOpenArrayPCRphotonicspolymerase chain reactionQProbe systemRochesingle nucleotide polymorphismSNPSNP genotypingswine fluSYBR Green 1Taq polymeraseTBthermal cyclingtuberculosisWeb Exclusives

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