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Andor Technology
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Conceptualizing Super-Resolution Microscopy eBook

Tuesday, November 21, 2023
A clever way to overcome the limitation caused by diffraction is to use 'pointillism strategy', a concept introduced by Rainer Heintzmann in 2005. The idea is to have only one fluorescent emitter (fluorophore) present at a given time for a given pixel. This localization leads to much higher resolutions. This idea was further developed into what is now known Single Molecule Localization Microscopy (SMLM).

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