Full company details
Applied Scientific Instrumentation Inc.
29391 W Enid Rd.
Eugene, OR 97402
United States
Phone: +1 541-461-8181
Fax: +1 541-461-4018
Toll-free: +1 800-706-2284
Multi-Immersion Objectives
ASI and Special
Optics have developed two dipping objective lenses designed for light sheet
microscopy of cleared tissue samples, including ASI’s ct-dSPIM. These
objectives work in any refractive index media without a correction collar
because of a unique curved first surface. They are robust to immersion in harsh
media including DBE and BABB. The original objective lens (54-10-12) has
nominal NA of 0.4, WD of 12 mm, and allows imaging over 5 mm deep into a flat
sample. The second one (54-12-8) has an increased NA of 0.7 with a WD of 10 mm.
Both NA and magnification vary slightly with the refractive index of the
immersion medium, but WD is constant. Like other ASI components, these
objective lenses are available for sale individually or as part of complete
systems. More objective lenses for light sheet microscopy are planned; inquire
for details. Applications: Dual-view cleared tissue imaging (diSPIM with
cleared tissues) Single-view cleared tissue imaging (OpenSPIM setups for
cleared tissues) Water-dipping applications with very long working distances.
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