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CHROMA TECHNOLOGY CORP.
Map10 Imtec Lane
Bellows Falls, VT 05101
United States
Phone: +1 802-428-2500
Fax: +1 802-428-2525
Toll-free: +1 800-824-7662

An Introduction to FRET, with an Emphasis on the Optics Involved

Monday, January 18, 2010
Fluorescent Resonant Energy Transfer (FRET) is a process that involves two fluorochromes (molecules that have the potential to fluoresce) with overlapping emission and excitation spectra. FRET is useful in determining intra-molecular and intermolecular reactions both spatially and temporally. It can be used to follow and record the interactions of proteins, enzymes, DNA, and RNA well below the 200 nanometer (10-9 meter) resolution limit of the optical microscope at its highest magnification and with the highest numerical aperture (NA) optics. In fact, FRET only allows resolution of interactions between 1 and 5nm, which is its beauty as well as its curse.

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