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Automation on a Budget

The light source for widefield fluorescence microscopy might seem like an unlikely enabler for high-speed live cell imaging, but the level of control that comes with solid-state LED Illumination Systems has changed the game.

Digital control via TTL capitalises on the maximum speed of LED switching, but often requires expensive specialist components. The CoolLED pE-300^ultra Illumination System (or the and pE-340^fura for calcium imaging) overcome this with two innovate features, enabling the capture of dynamic cellular processes with speeds of up to 10 µs. The ability to fit inline single-band excitation filters allows for a Pinkel configuration, removing the cost and latency of a filter wheel. The CoolLED Sequence Runner simplifies TTL control by utilising the TTL output signal available on most scientific cameras and cycles though LEDs in a user-selected sequence for each TTL signal.

This simple approach provides affordable high-speed microsecond switching precisely synchronised to the camera exposure, which also enhances data accuracy through minimised phototoxicity and photobleaching.

Microscopists can compare the speed, cost and signal-to-noise of typical configurations for automated Illumination Systems in a white paper available on the CoolLED website.More products