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Dual Light Sheet Microscopy

ASI’s Dual Selective Plane Illumination Microscopy for Cleared Tissue (ct-dSPIM) is one of many light sheet microscope configurations possible using our modular components. This flexible and easy-to-use Selective Plane Illumination Microscopy (SPIM) implementation allows for dual views of large samples such as cleared tissue (ct). We manufacture the optomechanical elements, including the motorized stages and 2D galvos for creating and moving the light sheet. ASI partnered with Special Optics to develop an objective optimized for light sheet imaging of cleared tissue.

Lasers and sCMOS cameras are required to complete the system; users can procure these themselves, use the services of various system integrators selling ASI SPIM systems, or purchase them via ASI. The ct-dSPIM has been successfully used to image various cleared tissue samples including whole mouse brains and slices of cleared tissue.

dSPIM Features:
   •  Image acquisition >10^8 voxels/sec
   •  Sub-micron resolution in XYZ (sample-permitting)
   •  Sample mounting in open dish
   •  Image >5 mm deep into flat samples or up to 12 mm radius sphere
   •  Media RI range from 1.33 to 1.56, aqueous or organic media
   •  Modular and flexible setupMore products