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Light Sheet Fluorescence Microscopy (LSFM)

Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates the specimen from the side in a single focal plane and is detected using a second objective oriented perpendicularly to the light sheet. By focusing light sheets onto a confined volume of the sample through cylindrical optics, optical sectioning and reduced phototoxicity are achieved.
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