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wide-field fluorescence microscopy

Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are activated by specific wavelengths of light and then emits a different wavelength. The microscope itself is set up with a light source (commonly an arc lamp, metal halide or LED) that emits the excitation light to the sample. The whole field of view is illuminated by the excitation light, hence the name, wide-field. The quality of the wide-field fluorescence is dependent upon carefully matched wavelengths from the light source, the filtration, the sample and the detector to the optimum excitation and emission peaks. The image's clarity is then determined by the quality of the filters, the magnification of both the objective and eyepiece and the numerical aperture (light collecting ability) of the objective.
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