Photonics Dictionary

A Ti:sapphire laser is a type of solid-state laser that utilizes a titanium-doped sapphire crystal as the gain medium. The name Ti:sapphire comes from the combination of titanium (Ti) as the dopant...

Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the principle of total internal reflection to selectively illuminate and image...

A transmission electron microscope (TEM) is a powerful microscopy technique that uses a beam of electrons to create high-resolution images of extremely thin samples. In a TEM, electrons are...

Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living tissue. The advantage of TPEF in comparison to conventional fluorescence...

An ultrafast pulsed laser is a type of laser that emits extremely short pulses of light with durations typically on the order of femtoseconds (10-15 seconds) or picoseconds (10-12 seconds). These...

Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet light, which is electromagnetic radiation with wavelengths shorter than...

A microscope used either to detect selective absorption of various wavelengths by the specimen or to achieve increased resolution by virtue of the short wavelength of ultraviolet radiation. Electron...

With respect to microscopy, light that is directed down through the objective onto the specimen and then returned by reflection.

With respect to microscopy, light that is directed through the specimen by a substage condenser.

Volumetric imaging refers to the capture, visualization, and analysis of three-dimensional (3D) information from a volume of space. Unlike traditional two-dimensional (2D) imaging, which provides...

A vortex phase plate is an optical device designed to impart a phase singularity, commonly referred to as a vortex or phase vortex, onto a light beam. This singularity results in a phase profile...

In microscopy, the clear distance between the specimen being viewed and the first optical element of the objective lens.

The removal of the zero-order component of the Fourier spectrum distribution of an object with a small, opaque absorber or reflector in the Fourier plane, to produce images that appear bright on a...