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Photonics Handbook
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Superresolution Microscopy: An Imaging Revolution
Marie Freebody, Contributing Editor
Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...
Fluorescence Lifetime Imaging: Choosing the Best Approach
GERHARD HOLST, Excelitas PCO GmbH
The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...
Lasers for Microscopy: Major Trends
Marco Arrigoni, Nigel Gallaher, Darryl McCoy, Volker Pfeufer, Matthias Schulze, and Daniel Callen, Coherent Inc.
Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...
Excimer Optics: High Power Demands High Reliability
Michael A. Case, Teledyne Princeton Instruments, Business Unit of Teledyne Technologies
The first high-reflectance mirror coatings for the UV and vacuum UV (VUV) were of the Al + MgF2 type produced in the late 1950s. Coatings of this design are still used today for multigas cavity...
Detectors: CCDs for Life-Science Applications
Butch Moomaw, Hamamatsu Corporation, Systems Div.
Since their invention in the late 1960s, charge-coupled devices, also called CCDs, have found widespread use in imaging applications. Electronic cameras based on CCD technology are used in...
Rules of Thumb
Photonics Rules of Thumb Scientists and engineers tend to want to answer simple relational questions with a blackboard covered with equations, even when questioners just want a rough estimate to...
Ultraviolet Reflectance Imaging: Applications
Dr. Austin Richards, Oculus Photonics
Reflected-ultraviolet imaging is a rather mysterious area of the imaging field. There is relatively little actual UV imagery to be found on the Internet or in the literature compared to near-infrared...
Spectroscopy: The Tools of the Trade
Dr. John R. Gilchrist, Clyde HSI
All optical spectrometry techniques rely on the measurement of radiant power. The configuration of the instrument varies based on the measurement technique: absorption, emission, luminescence, or...
NSOM: Discovering New Worlds
M. Kovar, Midako A. Nohe, N.O. Petersen and P.R. Norton, University of Western Ontario
NSOM is suitable for studies on the mesoscopic scale (several tens to hundreds of molecular dimensions). It has become an important tool in research and applications of semiconductors, organic layers...
Photonics Handbook
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