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26 terms

Photonics Dictionary: F

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fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of...
fluorescence correlation spectroscopy
A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in solution or membranes. FCS measurements rely on recording the transition of...
fluorescence decay system
A device, also called a fluorescence lifetime instrument, that measures decay curves of fluorescing samples. It generally consists of a gated pulsed flashlamp or a cavity dumped dye laser...
fluorescence lifetime
Fluorescence lifetime refers to the average time it takes for a fluorophore, a molecule that absorbs light at one wavelength and emits it at another, to return to its ground state after being excited...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of fluorescence emissions from fluorophores within a sample. Unlike traditional...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the...
fluorescence photography
The photographic recording of a subject that exhibits luminescence only for an extremely short time (10-8 s) after the exciting source is removed. In the process, the subject may be illuminated by...
fluorescence quenching
The suppression of fluorescence by absorption of the stimulating radiation.
fluorescence spectroscopy
The spectroscopic study of radiation emitted by the process of fluorescence.
FCS
fluorescence correlation spectroscopy
fiber optic imaging bundle
A fiber optic imaging bundle is a specialized optical device composed of multiple optical fibers bundled together. Each fiber optic strand within the bundle acts as a conduit for transmitting light....
fiber optic spectrometer
A fiber optic spectrometer is a device used for measuring the spectral content of light. It utilizes optical fibers to transmit light from a source to a spectrometer unit, where the light is...
fiber photometry
An optical recording technique that uses light source(s), a beamsplitting cube, light detector(s) and an optical fiber chronically implanted in animal brain to deliver excitation light to neurons...
FISH
fluorescence in situ hybridization
flame spectroscopy
The study of flames by means of a laser emitting blue light and a spectrometer to measure the green fluorescence created by the flame chemistry.
FLIM
fluorescence lifetime imaging microscopy
flow cytometry
Flow cytometry is a powerful technique used in biology and medicine for the quantitative analysis of the physical and chemical characteristics of cells and particles suspended in a fluid. The method...
fluorescein
Fluorescein is a synthetic organic compound extensively used as a fluorescent tracer in various applications, particularly in medicine and biology. It belongs to the xanthene dye family and is known...
fluorescent protein
Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a specific wavelength and emit light at a longer wavelength. These proteins are...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other forms of electromagnetic radiation, such as x-rays or ultraviolet light....
fluorometer
An instrument used to measure the duration of fluorescence emanating from a biological sample to monitor and evaluate its condition.
fluorometry
The analysis and measurement of the fluorescence emitted by a source. Fluorometric processes are more sensitive than light absorption methods and are rapidly being applied in medical and biological...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external energy source, such as ultraviolet or visible light. The process by which a...
fluoroscope
An instrument used to detect the fluorescence emitted by a source in comparison with a reference.
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced fluorescent molecules. This phenomenon is named after the German scientist...
FRAP
fluorescence recovery after photobleaching
Photonics DictionaryF

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