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Photonics Spectra
Mar 2005
Carl Zeiss Microscopy LLCRequest Info
LIVE-CELL IMAGING Carl Zeiss Jena GmbH has released a dedicated live-cell imaging system that collects up to 120 confocal images per second at a resolution of 512 × 512 pixels. The LSM 5 Live confocal microscope can attain 505 or 1010 fps at reduced resolutions of 512 × 100 or 512 × 50 pixels, respectively. Zoom optics and an ultrafast Z-drive permit 3-D image stacks to be acquired every second. A complex beam shaper converts the collimated Gaussian beam into laser light with a rectangular cross section and focuses it on the color-independent beamsplitter, which the company says provides nearly 100% excitation efficiency and emission yield at all wavelengths. Two individually adjustable confocal apertures enable maximum confocality at all levels.


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Gaussian beam
A beam of light whose electrical field amplitude distribution is Gaussian. When such a beam is circular in cross section, the amplitude is E(r) = E(0) exp [-(r/w)2], where r is the distance from beam center and w is the radius at which the amplitude is 1/e of its value on the axis; w is called the beamwidth.
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