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Fluorescence measurement

BioPhotonics
Apr 2009
Olympus Europa SE & Co. KG, Medical Systems & Micro-Imaging Solutions GroupRequest Info
 
Olympus Life Science Europa GmbH has announced that PicoQuant GmbH’s module for fluorescence lifetime imaging and fluorescence correlation spectroscopy can be fitted to the former company’s FluoView FV1000 confocal laser scanning microscope and FV1000MPE multiphoton ranges. The PicoQuant module is based on time-correlated single-photon counting using time-tagged time-resolved data handling. The addition of these two technologies, used to measure changes in fluorescence that occur in cells over short periods, increases the versatility of the microscope and ranges.


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GLOSSARY
fluorescence correlation spectroscopy
A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in solution or membranes. FCS measurements rely on recording the transition of several thousands of molecules through the focal volume. The combination of short measurement times along with free positioning or scanning of the observation spot makes FCS an excellent tool for investigating diffusion heterogeneity over time and space.
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