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Photonics Spectra
Nov 2009
PicoQuant GmbHRequest Info
PicoQuant.jpgPicoQuant GmbH has introduced version 1.2 software for use with its HydraHarp 400 time-correlated single-photon-counting system. The program provides a real-time correlator for fluorescence correlation spectroscopy (FCS) that calculates two autocorrelations and the cross correlation between two photon streams simultaneously. The new release supports Windows 7 and the latest HydraHarp hardware, with up to eight timing input channels. Each channel produces 1-ps resolution at a processing rate of 12.5 million counts per second per channel. All channels operate independently but with a common crystal time base. A time-tagged data collection mode provides a stream of individual timing events to the host computer that can be processed and analyzed for photon burst detection, coincidence correlation or for combined measurement of fluorescence lifetime and FCS.


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fluorescence correlation spectroscopy
A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in solution or membranes. FCS measurements rely on recording the transition of several thousands of molecules through the focal volume. The combination of short measurement times along with free positioning or scanning of the observation spot makes FCS an excellent tool for investigating diffusion heterogeneity over time and space.
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