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Fluorescence Detection

BioPhotonics
Jan 2010
Vertilon Corp.Request Info
 
Vertilon Corp. has introduced the SIB232 sensor interface board for fluorescence detection applications using the Hamamatsu H7260 multianode photomultiplier tube (PMT). In conjunction with the proprietary PhotoniQ data acquisition system, the board can simultaneously capture the 32 channels of high-speed, low-level fluorescence events detected by the H7260. The company’s latest circuitry for preamplification and discrimination of the PMT’s last dynode signal enables the fluorescence event itself to generate the trigger for data collection, without an external signal. Applications include flow cytometry, confocal microscopy and bioaerosol detection.


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GLOSSARY
discrimination
The degree to which a vision system is capable of sensing differences in light intensity between two regions.
flow cytometry
A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. Particles may be stained with fluorescent dye and the fluorescence detected via laser illumination.
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
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