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Microplate Reader

BioPhotonics
Mar 2010
Biochrom Ltd.Request Info
 
The Asys UVM340 Fastie-Ebert monochromator-based microplate reader has been introduced by Biochrom Ltd. The holographic grating enables users to select the exact wavelength, from 340 to 800 nm at 1-nm intervals, to measure optical density and absorbance for their applications, without the need to buy extra filters or change instruments to perform different assays. The single-channel reader is suitable for enzyme-linked immunosorbent assay and other absorbance applications, and it can measure any plate format from 12 to 384 wells. It reads a 96-well plate in 25 s. Front loading facilitates integration into automated systems, and the compact footprint saves bench space. The CE-marked reader uses a 30-W tungsten halogen lamp as the light source and delivers a bandwidth of <3 nm.


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GLOSSARY
absorbance
The natural log of the ratio of absorbed intensity over the total intensity which gives a constant value assuming a stable volume as well as energy. In optical physics the absorbance may be defined as the absorption cross section multiplied by the absorbing material length.
holographic grating
The generation of a grating on a blank by the holographic process. A series of interference fringes, corresponding to the grooves of the desired grating, are recorded on a photosensitive layer, and the subsequent chemical treatment forms a modulated profile on the surface of the blank by selective dissolution.
monochromator
Also known as monochromatic illuminator. An instrument for isolating narrow portions of the spectrum. The spectrum of any light source is formed by a prism or grating, and an exit slit placed in the spectrum selects a narrow band of wavelengths for emission. By moving the spectrum of a source internally past the slit, the color of the emitted light can be varied at will. As most monochromators emit several percentages of unwanted light -- either white or of the wrong wavelength -- along with...
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