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Fluorescent Conjugates

Apr 2010
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Vector Laboratories Inc. has added several DyLight fluorescent conjugates for immunofluorescent staining to its product range. Direct alternatives to traditional fluorophores, including fluorescein and rhodamine, the fluorescent dyes are conjugated to affinity-purified anti-mouse IgG (H+L), anti-rabbit IgG (H+L) and streptavidin. Current offerings include green DyLight 488, orange DyLight 549 and red DyLight 594. According to the company, all offer brighter fluorescence and greater photostability than other fluorochromes and can be easily substituted in existing protocols because of their parallel excitation and emission spectra. Stable over a pH range of 4 to 9, they are compatible with many aqueous-based buffers and diluents.


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1. The process by which an atom acquires energy sufficient to raise it to a quantum state higher than its ground state. 2. More specifically with respect to lasers, the process by which the material in the laser cavity is stimulated by light or other means, so that atoms are converted to a semistable state, initiating the lasing process.
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
The combination of the organic dye in a stained specimen and the antibodies produced that is detected by exposure to light.
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