BioSpectrum Imaging System
Jul 2010Analytik JenaRequest Info
UPLAND, Calif., July 12, 2010 — The BioSpectrum imaging system from UVP LLC is configured for use in multiple imaging applications. Combined with the BioLite multispectral light source, it provides a powerful system to excite and illuminate wavelengths for applications including multiplex near-IR imaging of protein blots. The system supplies a full range of wavelengths for excitation and enables rapid, high-resolution image capture through the use of a cooled CCD camera with low-light-sensitivity lenses that enables quick detection of a full range of visible and near-IR fluorescent dyes.
One-touch, pre-set or user-defined controls provide accurate, repeatable imaging and analysis for chemiluminescent, bioluminescent, fluorescent and colorimetric imaging. Other applications include 2-D gels and green fluorescent protein.
The company says that exposure times are faster than laser scanning and that the system is faster than film for western blot imaging.
Features include easy-to-use VisionWorks LS 7.0 software that automates research with templates for consistent, reproducible results; automated imaging of white light and multiple different fluorescent tags on a single blot; and a combination of up to eight excitation and five emission wavelengths to accommodate all spectral ranges in a single experiment.
Other benefits include motorized optics for setting the aperture, zoom and focus; automated templates for repeat experiments; a lighttight darkroom for optimum imaging conditions; a UV-blocking viewing window for quick sample inspection; and automated control of the five-position emission filter array.
With the extensive range of excitation and emission filters available, researchers can detect as well as quantify virtually all fluorescent dyes from the visible to the near-IR using the acquisition and analysis software. The use of near-IR and visible fluorescent labels permits multiplexing so that several proteins in a sample can be detected and analyzed at the same time on a single protein blot.
The company offers several filters used for near-IR blotting with 680- and 770- to 800-nm fluorescent tags. Routine near-IR imaging is fast, efficient and straightforward, yielding full 16-bit images for quantitation and publication.