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Combined Camera for Fluorescence Spectrometers
Feb 2013
Edinburgh Instruments Ltd., PhotonicsRequest Info
LIVINGSTON, Scotland, Feb. 14, 2013 — Edinburgh Instruments Ltd.’s Photonics Div. has added a combined microscope and imaging camera to its FLS980 and FLS920 photoluminescence spectrometers. The microscope-camera combination enables measurement of specific points within a sample and allows imaging with monochromatic excitation/illumination.

Upright and inverted microscopes from leading manufacturers can be coupled to the spectrometers via optical fibers. Imaging is possible with wide-field excitation or in spot mode with approximately 1-μm spatial resolution. Cameras are available for the visible and near-infrared regions up to 1700 nm, and all microscopes and cameras can be retrofitted to the spectrometers.

The FLS980 and FLS920 photoluminescence spectrometers use single-photon-counting techniques for steady-state and lifetime measurements in fluorescence and phosphorescence. The spectrometer software provides full control of the microscope, including automated microscope stage scanning and data acquisition for mapping.

A choice of configurations provides measurement versatility. Spectral scanning of large areas is possible using wide-field epi-illumination, or of much smaller areas using lasers for excitation. Fluorescence and phosphorescence time-resolved measurements can also be made in wide-field or spot mode.


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The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
Luminescence is the emission of light from a source that is delayed by more than 10-8 s following excitation.
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