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NFM SWIR Fluorescence Microscope

BioPhotonics
Feb 2014
Applied NanoFluorescence LLCRequest Info
 
HOUSTON, Dec. 3, 2013 — The NFM microscope from Applied NanoFluorescence images individual single-walled carbon nanotubes (SWCNTs) through their intrinsic short-wave IR fluorescence. The system is suitable for research applications in the fields of physics, chemistry, biomedicine and the environment.

Each 915 × 610-mm system includes a modular inverted fluorescence microscope; optical filters for SWCNT excitation and imaging; an LS-4 fluorescence excitation system with four computer-controlled lasers; optics for controlling excitation beam polarization, intensity and focusing; and a thermoelectrically cooled scientific-grade InGaAs camera for image capture. A short-wave IR spectrometer for fluorescence microspectroscopy is optional.


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GLOSSARY
fluorescence microscopy
Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction. 
single-walled carbon nanotubes
Referred to as SWCNTs, these cylindrical nanostructures composed of a folded sheet of graphene can be used as near-infrared markers for small animal multiplex imaging. Emitting at different wavelengths, which can be tuned in the visible or NIR region, SWNCTs provide photostable, narrow bandwidth fluorescence in a wide chromatic variety. That enables simultaneous imaging of a large number of spectrally separated markers, through the use of global hyperspectral imaging. In addition to being used...
spectrometer
A kind of spectrograph in which some form of detector, other than a photographic film, is used to measure the distribution of radiation in a particular wavelength region.  
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