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Laser Scanner

BioPhotonics
Jun 2014
Applied Scientific Instrumentation Inc.Request Info
 
EUGENE, Ore., Feb. 19, 2014 — The Fiber-Coupled Laser Scanner by Applied Scientific Instrumentation is a 2-D galvo unit designed for generating SPIM (selective plane illumination microscopy) light sheets. It is also suitable for optogenetics, local uncaging and fluorescence recovery after photobleaching (FRAP).

A light beam enters the scanner on a standard fiber optic connector. In the sample plane, the beam is steered with an electronically controlled galvo comprising an integrated two-axis MEMS mirror. An optional anti-striping mirror varies the beam’s angle of incidence on the sample to mitigate shadowing effects. Adjusting the iris changes the light sheet thickness and depth of focus. By steering the beam to one corner, it can effectively be turned off or “blanked.”

A C-mount port connects the scanner to the microscope.


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GLOSSARY
optogenetics
A discipline that combines optics and genetics to enable the use of light to stimulate and control cells in living tissue, typically neurons, which have been genetically modified to respond to light. Only the cells that have been modified to include light-sensitive proteins will be under control of the light. The ability to selectively target cells gives researchers precise control. Using light to control the excitation, inhibition and signaling pathways of specific cells or groups of cells...
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