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Light-Sheet Imaging

Mar 2015
Nikon Instruments Inc.Request Info
MELVILLE, N.Y. — The new Ti-diSPIM module from Nikon Instruments Inc. gives light-sheet imaging capabilities to the company’s flagship Ti inverted research microscope.

Selective plane illumination microscopy (SPIM) minimizes phototoxicity and photobleaching in imaging of live specimens. A thin sheet of light is used to illuminate a focal plane of the cell that is coincident with the focus of the imaging lens. This also provides optical sectioning by not illuminating out-of-focus regions, which would normally result in image blur.

The module integrates Applied Scientific Instrument’s dual-inverted SPIM hardware for rapid acquisition of two different views of the sample to generate 3-D images with isotropic resolution.

Nikon also offers two different objective lenses for SPIM. The 40× 0.8 NA provides up to 300-nm resolution, while the 10× provides a larger field of view.


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