Jun 2015Lambert Instruments BVRequest Info
RODEN, Netherlands — A new fluorescence lifetime imaging microscopy (FLIM) system from Lambert Instruments BV is compatible with any wide-field fluorescence microscope.
The LIFA-TD (Lambert Instruments FLIM attachment for time-domain imaging) includes a camera with a built-in image intensifier, which can reduce the exposure time to 3 ns and alter timing of the exposure in the subnanosecond range. A pulsed laser excites the fluorophores in the sample. By using a laser with a small pulse width, the accuracy of lifetime measurements is optimized.
Images are recorded with custom software, which also processes the data to determine fluorescence lifetime. The fluorescence lifetimes are then shown as a colorized overlay on the original image.