Versatile Picosecond Laser Module
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Externally triggerable high power picosecond laser for STED depletion. PicoQuant's new VisIR-765 “STED” is an externally triggerable high power laser at 766 nm with optimized pulse-width for STED depletion.
The laser VisIR-765 “STED” delivers an optical output power of up to 500 mW at 766 nm in a collimated output beam with a laser pulse width of 300 to 500 ps. The laser can be operated at 12 different internally selectable repetition rates between 80 MHz and 31.25 kHz and can also be triggered externally by TTL or NIM signals at any repetition rate between 1 Hz and 80 MHz. The laser is based on a gain switched seed laser diode at 1532 nm combined with a multi-stage fiber amplifier and a SHG-stage for the generation of the second harmonic at 766 nm. The VisIR-765 “STED” is a stand alone device including all driving functions of the classical PDL series laser driver from PicoQuant. The optional remote control allows to set the trigger source, the repetition rate, and the general output power of the laser.
The VisIR-765 “STED“ is ideally suited to be used as a depletion laser for stimulated emission depletion (STED) microscopy. The relatively long pulse widths of several hundred picoseconds permit to obtain high resolution images and minimizes fluorescence photobleaching, since longer pulses or even continuous-wave excitation can expose the sample to an unnecessary amount of radiation that can lead to increased photobleaching.