Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a very versatile microscopy method where fluorescence lifetime information is combined with spatial localization in the sample, allowing investigating, for example, biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition is typically based on Time-Correlated Single Photon Counting (TCSPC) electronics, pulsed diode lasers, and highly sensitive single photon counting detectors. Up to now TCSPC...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. The integration can overlay both FLIM and Raman images...Promoted Content April 2024 IssueGet More Data: Automate Your MicroscopeZaber offers a selection of standard and customized motorized microscope stage products. They offer: Exceptional Performance Maximize your throughput and consistency with up to 500-nm...Vision Spectra Summer 2023 IssueSWIR CamerasThe Triton™ SWIR IP67-rated 1.3- and 0.3-MP cameras from LUCID Vision Labs Inc. are compact, cost-efficient devices featuring Sony SenSWIR InGaAs sensors. The GigE Power over Ethernet...Photonics Spectra May 2024 Issue3D Measuring DeviceThe FocusX from Bruker Alicona is an optical 3D measuring device meant for applications where highly reflective surfaces, steep flanks, and complex forms must be measured. Able to measure small...ARTICLESBioPhotonics Nov/Dec 2022 IssueFlow Cytometry Technique Shows Promise for Early Cancer DiagnosisA digital droplet microfluidic flow cytometry technique could enable earlier recognition of tumor development and progress, by aiding in the detection of multiple microRNAs (miRNAs) in single...BioPhotonics May/Jun 2023 IssueStress tests borne of mice and menMen and women respond differently to stimuli, and their hearts respond differently, too. Scientists are continuing to develop an understanding as to why these disparities in physical reactions exist....Photonics.com 3/14/2023Graphene Properties Add Axial Resolution Precision to 3D ImagingResearchers at the Ludwig Maximilian University of Munich (LMU) combined pulsed-interleaved MINFLUX (pMINFLUX) nanoscopy with a newly developed method for axial resolution that exploits the special...BioPhotonics Sep/Oct 2022 IssueA Multiphoton Microscope Enables Portable 3D Biological ImagingFor decades, optical microscopy has provided the mechanism with which to image cells and tissue for the purposes of cancer research, digital pathology, and the study of the brain. Samples are...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookSuperresolution Microscopy: An Imaging RevolutionSuperresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookHigh-Speed Video: Selecting a Slow-Motion Imaging SystemThere is a growing market for imaging systems that provides an immediate, slow-motion view of a process that allows one to see events that happen too quickly for the human eye to perceive or...Photonics HandbookHyperspectral Imaging Spectroscopy: A Look at Real-Life ApplicationsHyperspectral imaging spectroscopy has developed dramatically from a large, complex, remote-sensing satellite- or aircraft-based system into a rugged, compact, economically priced imaging and...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 2/28/2024Multi-Bandpass Filters for FluorescenceLearn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.Photonics.com 9/27/2023Raman SpectroscopyTo achieve accurate and precise Raman measurements, optical filters play a crucial role in enhancing signal-to-noise ratio, and reducing background interference. This white paper explores the...Photonics.com 2/21/2024Overview of Photon etc’s Laser Line Tunable Filter (LLTF) and Tunable Laser Source (TLS) applicationsThe Laser Line Tunable Filter (LLTF) is a non-dispersive, widely tunable bandpass optical filter. At its core lies a volume Bragg grating (VBG) which efficiently selects and filters all but a narrow...WEBINARSPhotonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. Emphasis is placed on the integration of FLIM in surgical...Photonics.com 11/20/2019Frequency-Domain Fluorescence Lifetime Imaging: System Improvements and ApplicationsSince the introduction of the first frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) pco.flim camera in 2014, this camera system has been applied to many different areas, from FRET...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarysingle-photon avalanche diodeA single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical signals, down to the level of single photons. SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionarylight sheet fluorescence microscopyAlso known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates...Photonics Dictionaryintensified charge-coupled device cameraAn intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an image intensifier and a charge-coupled device (CCD) sensor to achieve...VIDEOSPhotonics.com 10/12/2021pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 1/18/2018Supporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 11/20/2023Andor's Life Science Research SolutionsAt Andor, it matters to us that the products we develop make a real difference to our customers and contribute significantly to their research across Immunology, Microbiology, Neuroscience, Cells &...PHOTONICS BUYERS' GUIDE CATEGORIESAutocorrelatorsAn autocorrelator is a signal-averaging device that improves signal-to-noise ratio by comparing a sampled signal with a time-delayed form of itself.Streak CamerasA camera streak is a high-speed cine camera used to record, on a continuously moving film, very brief events such as a flash of light. The image is recorded as a continuous image, not as a series of...Picosecond LasersUltrafast Pulsed LasersAn ultrafast laser is a type of laser that emits extremely short pulses of light with durations typically on the order of d(10^-15 seconds) or picoseconds (10^-12 seconds). These lasers are capable...COMPANIESPhotonics Buyers' GuidePhotron USA Inc.Photron has been a global market leader in high-end, high-speed camera systems for over forty years. The company’s continuing development of new state-of-the-art products illustrates a...Photonics Buyers' GuideLaserPoint srlLaserPoint manufactures a wide portfolio of laser power and energy meters able to perform laser measurements with high accuracy. They developed the hard coatings to absorb the laser radiation which...Photonics Buyers' GuideDK Photonics Technology LimitedDK Photonics is a leading manufacturer of innovative and high quality optical passive components. We provide complete and integrated solutions from 400~2100nm. DK Photonics produces a large array of...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...