Request InfoPicoQuant GmbHFluorescence Lifetime Imaging (FLIM) is a versatile microscopy method, which combines fluorescence lifetime information with spatial localization, enabling the investigation of biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster Resonance Energy Transfer (FRET). FLIM data acquisition based on Time-Correlated Single Photon Counting (TCSPC) is considered a somewhat slow process, due to the time required to collect a sufficient number of photons per pixel for reliable data analysis. ...See full productRelated content from Photonics MediaPRODUCTSBioPhotonics Jul/Aug 2023 IssueFluorescence Lifetime Imaging Microscopy ModuleA fluorescence lifetime imaging microscopy (FLIM) module has been added to Renishaw PLC’s inVia™ confocal Raman microscope. The integration can overlay both FLIM and Raman images...BioPhotonics May/Jun 2023 IssueMonochrome Microscope CameraThe Digital Sight 50M monochrome microscope camera from Nikon Instruments Inc. conducts high-precision analysis and large-volume sampling for use in applications such as well plate screening. ...BioPhotonics Mar/Apr 2024 IssueMicroplate WellsThe series of wells that have been added to ibidi’s µ-Plates include an expansion to its µ-Plate 96 Well to include both square and round shapes. The plates are available in 24, 96,...BioPhotonics Sep/Oct 2023 IssueUpright MicroscopeThe ECLIPSE Ui integrated microscope-based viewing system from Nikon Instruments Inc. is designed for clinical and pathological observation. The system provides a digital user experience,...ARTICLESPhotonics.com 6/27/2022Perovskite Cell, Test Method Set High Bar for Advancing TechnologyResearchers at Princeton University have developed a perovskite solar cell device that can perform above industry standards, maintaining operation for approximately 30 years — compared to the...Photonics.com 2/16/2024Nanocavity Approach Enables Unprecedented Confinement, LifetimeResearchers from The Institute of Photonic Sciences (ICFO) have introduced a type of polaritonic cavity that redefines the limits of light confinement. The work demonstrates an unconventional way of...Vision-Spectra.com December 2023 IssueVisual Components Launches Joint Venture with Mitsubishi Electric3D manufacturing technology company Visual Components has formed a joint venture with longtime collaborator Mitsubishi Electric. The venture, called ME Industrial Simulation Software, seeks to...Photonics.com 2/23/2024SPAD Elevates Spatiotemporal Resolution in Conventional MicroscopesImage scanning microscopy (ISM), a superresolution technique enabled by the advent of fast and compact detector arrays, provides signal-to-noise ratio (SNR), optical sectioning, and spatial...PHOTONICS HANDBOOK ARTICLESPhotonics HandbookFluorescence Lifetime Imaging: Choosing the Best ApproachThe term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookLasers for Microscopy: Major TrendsLaser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...Photonics HandbookMicroscopy Illumination: Considering Nonlaser Light SourcesAs recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...Photonics HandbookAspheric Lenses: Design ConsiderationsAspheric surfaces are powerful tools that combine the optical corrections of multiple lenses into a single element (Figure 1) and affect performance in ways that spherical optics cannot. For example,...WHITEPAPERSPhotonics.com 10/19/2022Multiphoton Imaging of Mouse IntestineTwo-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared...Photonics.com 4/20/2023Is a DIY Microscope for You?For some advanced microscopy and nanoscopy techniques it becomes necessary to modify existing instrumentation or build an instrument from scratch. This article helps readers determine whether a...Photonics.com 9/27/2023Raman SpectroscopyTo achieve accurate and precise Raman measurements, optical filters play a crucial role in enhancing signal-to-noise ratio, and reducing background interference. This white paper explores the...Photonics.com 1/10/2024Single-Objective Light SheetAn overview of the Single-Objective Light Sheet.WEBINARSPhotonics.com 5/19/2020Squeezing More Out of Light: Innovative Approaches to Time-Resolved Flow CytometryIn this webinar, presenter Giacomo Vacca, Ph.D., founder and president of Kinetic River, will discuss recent advances in time-resolved methods of flow cytometry and how these advances are being...Photonics.com 4/14/2016FLIM (Fluorescence Lifetime Imaging in the Frequency Domain) in a NutshellJoin us for a free webinar presented by PCO! Due to advancements in CMOS image sensor technology a new camera system, pco.flim, has been created to allow for easy and fast fluorescence (and...Photonics.com 3/21/2013Techniques in Biophotonic ImagingFREE WEBINAR Sponsored by: Hamamatsu & Applied Scientific Instrumentation Additional Questions & Answers from the webinar are below: Dr. Kimani C. Toussaint Jr. Quantitative...Photonics.com 10/26/2022KEYNOTE: FLIM in Surgical OncologyMarcu reviews the development of clinically compatible fluorescence lifetime imaging (FLIM) technology and applications in surgical oncology. Emphasis is placed on the integration of FLIM in surgical...PHOTONICS DICTIONARY+ TERMSPhotonics Dictionarysingle-photon avalanche diodeA single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical signals, down to the level of single photons. SPADs are widely used in...Photonics Dictionarytime-correlated single photon countingTime-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging...Photonics Dictionarytime-to-amplitude converterA time-to-amplitude converter (TAC) is an electronic circuit that converts a time interval between two events into an amplitude or voltage signal. TACs are commonly used in various scientific and...Photonics Dictionaryfluorescence lifetime imagingFluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of fluorescence emissions from fluorophores within a sample. Unlike traditional...VIDEOSPhotonics.com 10/12/2021pco - Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On SessionThis hands-on session about FD-FLIM by Gerhard Holst from PCO AG was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences".Photonics.com 11/20/2023Andor's Life Science Research SolutionsAt Andor, it matters to us that the products we develop make a real difference to our customers and contribute significantly to their research across Immunology, Microbiology, Neuroscience, Cells &...Photonics.com 11/20/2023New ZL41 Cell & Sona sCMOS - Easy, Flexible, Cost-Effective Super Resolution Microscopy - Andor TechnologyEasy and Cost-Effective Super-Resolution to ~100 nm. Andor has introduced two new highly innovative sCMOS cameras, both delivering easy and cost-effective access to super-resolution capabilities and...Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...PHOTONICS BUYERS' GUIDE CATEGORIESPicosecond LasersStreak CamerasA camera streak is a high-speed cine camera used to record, on a continuously moving film, very brief events such as a flash of light. The image is recorded as a continuous image, not as a series of...AutocorrelatorsAn autocorrelator is a signal-averaging device that improves signal-to-noise ratio by comparing a sampled signal with a time-delayed form of itself.Stroboscopic Light SourcesA stroboscopic light source is an electronic flash tube capable of repeated operation at hundreds or thousands of flashes per second for long periods.COMPANIESPhotonics Buyers' GuidePhotron USA Inc.Photron has been a global market leader in high-end, high-speed camera systems for over forty years. The company’s continuing development of new state-of-the-art products illustrates a...Photonics Buyers' GuideLaserPoint srlLaserPoint manufactures a wide portfolio of laser power and energy meters able to perform laser measurements with high accuracy. They developed the hard coatings to absorb the laser radiation which...Photonics Buyers' GuideDK Photonics Technology LimitedDK Photonics is a leading manufacturer of innovative and high quality optical passive components. We provide complete and integrated solutions from 400~2100nm. DK Photonics produces a large array of...Photonics Buyers' GuideAndor TechnologyAndor Technology Ltd is a global developer and manufacturer of high-performance scientific cameras, microscopy systems and spectrographs for academic, government, and industrial applications. Founded...