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A-TEEM and Molecular Fingerprinting

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A-TEEM and Molecular FingerprintingA-TEEM (Absorbance and Transmission Excitation Emission Matrix) simultaneously measures absorbance and fluorescence transmission. A-TEEM fingerprints molecules with high specificity and ultrahigh-sensitivity enabling researchers to identify, quantify and understand dynamics of fluorescing and absorbing molecular states, and mixtures. A-TEEM is found only on the Aqualog, which is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC. A-TEEM acquisition and analysis timeframe takes place in seconds to minutes.

Aqualog® combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with new A-TEEMTM technology that uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh -sensitivity at a 6 million nm/min emission scan rate! A-TEEM has already proven to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy. Knowledge of the presence, identity, and concentrations of amino acids during cell culture processes is critical for optimizing nutrient levels for better cell growth and improved product yield.


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ProductsHoriba ScientificspectroscopyEEMsfluorescencemolecular fingerprintingabsorbanceAqualogCDOMfluorometerproteinvaccinewine researchwater researchwater treatment

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