Calibration Slide

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Calibration SlideDeveloped with input from bright-field microscopy experts, the IAM-9C-SECCAL color transmission calibration slide from Applied Image Inc. offers NIST-traceable color integrity of digitally captured microscopy images.

The slide contains 24 colors in a 4 × 6 array, similar to the MacBeth color checker. Each of the 24 4150-μm-diameter patches is individually calibrated. Smaller patches from 1000 to 150 μm are exposed and processed at the same time to produce good correlation of the large calibrated patches to the smaller ones. A user needs only to take an image of one of the arrays in order to use the calibration data provided for the slide.

The color calibration slide is manufactured, serialized and individually calibrated utilizing a state-of-the art spectrophotometer. The spectral transmission response (%T) from 340 to 830 nm is recorded for each color patch and reported in 5-nm increments. This data allows users to calibrate imaging systems and color correct images taken.

A black background minimizes flare and a large clear area is used to establish a baseline transmission through glass, making the slide versatile across many applications. With a standard 0.15-mm glass cover slip permanently cemented over the color film, the slide can be used with oil immersion objectives.

The product is software-agnostic, allowing data to be converted to the desired color space, such as Adobe RGB 1998, L*A*B*, XYZ, etc., utilizing a supplied spreadsheet.

Included with the product are the calibrated IAM-9C-SECCAL color microscope slide, a calibration certificate, calibration data report, conversion spreadsheet and a USB flash drive with data and spreadsheet, all in a protective wooden case.

Published: February 2018
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ProductsApplied Image Inc.calibration slideMicroscopyBiophotonicsImagingarraysspectrophotometerIAM-9C-SECCALcolor transmission calibration slideNIST

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