Elyra P.1 3-D Superresolution Microscopy

Carl Zeiss Spectroscopy GmbHRequest Info

Zeiss has added the Elyra P.1 module to its 3-D microscopy portfolio. The system enables superresolution photoactivated localization microscopy (PALM) for endogenously expressed photoswitchable fluorescent proteins. This capability allows researchers to investigate the structural arrangement of one or multiple proteins, reveal the structure of organelles in 2-D and 3-D, and count and map molecules within a structure.

In PALM, photoswitchable fluorescent molecules are sparsely activated so that only one out of many will be in its “on” state within a single point spread function (PSF). In 3-D, the PSF shape codes for the Z-position. The localizations are plotted in a new image to create the superresolved image. Elyra P.1 achieves resolutions in the range 20 to 30 nm laterally and 50 to 80 nm axially.

The system can be integrated with various proprietary laser scanning and scanning electron microscopes.

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BioPhotonics
Feb 2014

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Elyra P.1 3-D Superresolution Microscopy