FV10-ASW 3.0 Software

Olympus Europa SE & Co. KG

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HAMBURG, Germany, June 8, 2011 — Olympus Europa Holding GmbH has introduced FV10-ASW 3.0, the latest version of its software for the FluoView FV1000 range of confocal laser scanning microscopes and Fluoview FV1000MPE multiphoton excitation systems.

Incorporating high-dynamic-range imaging (HDRI) results in a greater dynamic range of fluorescence between the lightest and darkest areas of a sample. Signal-to-noise ratios are minimized, enabling capture of clear, highly resolved images. Additional functionality, such as partial stitching with multiarea time-lapse imaging, and channel unmixing for the FluoView FV1000MPE systems, provides flexibility for obtaining high-quality images. The new software is fully compatible with Microsoft Windows 7.

As an advanced image composition technique, HDRI provides users with a tool to produce images with good contrast and no saturation. Merging multiple images captured under different exposure settings results in a final image that provides good clarity, with the ability to visualize even the more challenging cellular components.

Version 3.0 allows users to select specific areas of the whole sample, which can be chosen and stitched together easily. This time-saving function is key to performing quick and simple analysis of specific regions of interest.

With advances for the FV1000MPE systems, the non-descanned detectors, which enable users to cover every multiphoton excitation requirement and acquire maximum fluorescence output, can now also be used for channel unmixing. Users can perform spectral unmixing of multicolor samples, even those with significant spectral emission crossover. A laser limiter sets upper intensity boundaries to avoid overstimulation of sensitive samples, ensuring that cellular integrity is maintained.

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Published: June 2011