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Illumination Microscopy

Leica Microsystems GmbH
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WETZLAR, Germany, May 27, 2010 — Resolution and contrast are crucially important for fluorescence applications in wide-field microscopy. Undesired haze effects that reduce contrast can be minimized by using the smart principle of structured illumination, which results in fluorescence images with good contrast, high axial resolution and ultrasharp 2-D sections of the specimen.

Intelligent Structured Illumination Microscopy from Leica Microsystems uses the proven OptiGrid technique. Working in harmony with the company’s high-quality optics, the aperture diaphragm integrated with the fluorescence light path also enhances contrast. The automatic focus function of the OptiGrid keeps the grid structure in focus, from UV to IR light.

The same OptiGrid module can be used both for upright and inverted Leica research microscopes, and there is no need to change the grid because one optimized grid covers all magnifications from 10× to 100×, offering convenience, avoiding errors and saving valuable time.

Intelligent Structured Illumination Microscopy provides a complete system from a single source: The OptiGrid module integrated with Leica research microscopes is controlled by intuitive and proprietary MM AF software and complemented by a wide selection of fluorescence cameras.
May 2010
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aperture diaphragmautomatic focusBiophotonicsEuropefluorescencefluorescence light pathIntelligent Structured Illumination MicroscopyinvertedIRLeica MicrosystemsMicroscopyopticsOptiGridProductsSoftwareuprightUVwide-field microscopy

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