Light Sheet Microscope

Intelligent Imaging Innovations Inc. (3i)Request Info

The Cleared Tissue LightSheet from Intelligent Imaging Innovations Inc. is a light-sheet microscope that can image whole organs at a high speed by illuminating them with structured light using high-resolution spatial light modulators.

Similar to other fluorescence microscope techniques, the Cleared Tissue LightSheet uses laser light to stimulate fluorescence emission from genetically labelled tissue to form an image of the specimen. The microscope creates a finely focused sheet of light for optical sectioning, then uses the modulator to rapidly shift the thinnest portion of the sheet in synchronization with the microscope and camera, resulting in ultra-fast and high-resolution imaging of large specimens. Piezoelectric stages move the specimen in X, Y, and Z directions.

The emissions from the fluorescent markers in the specimen are recorded by a camera, with software automatically and precisely reconstructing the biological structure in 3D for further analysis.

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BioPhotonics
Nov/Dec 2020

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Light Sheet Microscope