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Light Sheet Microscopy (oSPIM)

Applied Scientific Instrumentation Inc.Request Info
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Applied Scientific Instrumentationnnnn's Light Sheet Microscopy (oSPIM)ASI’s Oblique Single Illumination Microscope (oSPIM) is an excellent platform for high resolution light sheet microscopy for samples mounted in standard coverslip-bottom culture dishes. The oSPIM is a single-view light sheet system where the illumination light sheet is generated at an oblique angle using an oil immersion objective below the sample dish. Fluorescent emission is observed using a high NA water dipping objective from the top side where this objective is tilted 60 degrees, perpendicular to the illumination sheet. The arrangement with high NA objectives both above and below the sample dish allow for high-resolution imaging in a convenient geometry for cell culture work.

The oSPIM is two microscopes in one. The lower microscope can be used for fluorescent imaging using conventional modalities, such as wide field fluorescence, confocal, or TIRF. It also is utilized as the light sheet excitation objective. The tilted top microscope is dedicated to Selective Plane Illumination Microscopy (SPIM) or light sheet images.

oSPIM Features:

   •   Low photobleaching: >10x reduction vs. confocal/spinning disk
   •  Water dipping emission objective yields 280 nm transverse resolution
   •  Rapid 3D imaging at oblique angle to coverslip, up to 200 planes per second
   •  Conventional sample mounting in cell culture dishes
   •   Fully functional “conventional” fluorescent microscope in addition to the light sheet modality
   •  Modular and flexible


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ProductsBiophotonicsApplied Scientific InstrumentationMicroscopyHigh Resolution Light SheetRapid 3D Imagingcell cultureSelective Plane Illumination

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