Light Sheet for Cleared Tissue
Applied Scientific Instrumentation Inc.Request Info
The ct-dSPIM is a flexible and easy-to-use light sheet microscopy configuration optimized for imaging large cleared tissue samples. The sample is mounted on a motorized XYZ stage and imaged via stage scanning. Two multi-immersion or other objective lenses are held in an upright “V” geometry for light sheet illumination and detection. Acquisition speed is usually camera-limited.
ASI manufactures the optomechanical elements, including the motorized stages and 2D galvos for creating and moving the light sheet. Lasers and sCMOS cameras are required to complete the system; users can procure these themselves, use the services of various system integrators selling ASI SPIM systems, or purchase them via ASI. The ct-dSPIM has been successfully used to image various cleared tissue samples including whole mouse brains and slices of cleared tissue.
ct-dSPIM Features
Image acquisition >10^8 voxels/sec
Sub-micron resolution in XYZ (sample-permitting)
Sample mounting in open dish
Image >5 mm deep into flat samples or up to 12 mm radius sphere
Media RI range from 1.33 to 1.56, aqueous or organic media
Modular and flexible setup
http://www.asiimaging.com/products/light-sheet-microscopy/dual-selective-plane-illumination-microscopy-for-cleared-tissue/
https://www.photonics.com/Buyers_Guide/Applied_Scientific_Instrumentation_Inc/c1021
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