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Light Sheet for Cleared Tissue

Applied Scientific Instrumentation Inc.Request Info
 
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Light Sheet for Cleared TissueThe ct-dSPIM is a flexible and easy-to-use light sheet microscopy configuration optimized for imaging large cleared tissue samples. The sample is mounted on a motorized XYZ stage and imaged via stage scanning. Two multi-immersion or other objective lenses are held in an upright “V” geometry for light sheet illumination and detection. Acquisition speed is usually camera-limited.

ASI manufactures the optomechanical elements, including the motorized stages and 2D galvos for creating and moving the light sheet. Lasers and sCMOS cameras are required to complete the system; users can procure these themselves, use the services of various system integrators selling ASI SPIM systems, or purchase them via ASI. The ct-dSPIM has been successfully used to image various cleared tissue samples including whole mouse brains and slices of cleared tissue.

ct-dSPIM Features   
  • Image acquisition >10^8 voxels/sec   
  • Sub-micron resolution in XYZ (sample-permitting)   
  • Sample mounting in open dish Image >5 mm deep into flat samples or up to 12 mm radius sphere
  • Media RI range from 1.33 to 1.56, aqueous or organic media   
  • Modular and flexible setup


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    ProductsMicroscopylight sheet microscopyCleared Tissue SamplesDual Selective Plane Illumination MicroscopySub-Micron Resolutionmulti-immersion objectivesSPIMdSPIM

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