Lightning-Fast LED Illumination

CoolLED Ltd.

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CoolLED - Lightning-Fast LED Illumination

CoolLED's ground-breaking pE-800 Series Illumination Systems for fast, powerful, and affordable imaging is popular for high-speed live cell fluorescence — but the potential applications don't end there. While the pE-800 fura includes 340- and 380-nm LEDs for Fura-2 calcium imaging, both the pE-800fura and pE-800 are ideal for further applications including:

FRET
Spectroscopic imaging of chromogens
Ratiometric fluorescence
High-content imaging
Spatial biology
FISH
pH monitoring
Calcium imaging (ratiometric and single indicators)
Optogenetic stimulation
Spinning disk confocal
Light-hungry applications

Both the pE-800 and pE-800fura feature eight powerful and efficient LEDs for broad spectral coverage and individual channel control. While the pE-800 covers fluorophores from DAPI to Cy7 (365 - 740 nm), the pE-800fura is ideal for scientists primarily researching calcium, covering Fura-2 to Cy5 (340 - 635 nm).

Lightning-fast <7 µs TTL triggering is ideal for capturing dynamic events or enhancing accuracy of ratiometric measurements — for example with Fura-2 calcium imaging. Moreover, reduced latency saves time during applications such as image tiling and when high throughput is vital. For live cell imaging, synchronizing the light source and camera also minimizes photobleaching and phototoxicity for more accurate data.

Scientists can benefit from analogue control and support in several imaging software platforms, including Evident cellSens, Nikon NIS Elements, and µManager; while the intuitive CoolLED LightBridge GUI offers additional control options.

For even more specialized applications, the pE-800 Series can also be customized for OEM requirements. Versatile, simple to use and backed by CoolLED's world-renowned support and a generous warranty, the pE-800 Series makes next generation LED illumination accessible to all.

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