Live-Cell Fluorescence Imaging

Olympus Europa SE & Co. KGRequest Info
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HAMBURG, Germany, March 2, 2010 – Olympus Europa Holding GmbH has introduced its cell^FRAP system for fluorescence recovery after photobleaching (FRAP), fluorescence loss in photobleaching, pattern bleaching to simulate fluorescent speckle microscopy, photoactivation and photoconversion experiments.

It performs millisecond real-time photocontrol and photomanipulation with hardware-based device synchronization. Intelligent scanning algorithms that target only the region of interest (ROI) remove the need for line scanning between ROIs and increase scan speed. Simultaneous photobleaching and image acquisition of multiple ROIs are possible.

The system offers predefined FRAP experiments for investigation of cellular dynamics, and “fire on click” experiments enable online triggering of single- and multipoint bleaching.

The system uses a real-time controller and camera-based imaging. The first frames can be acquired as early as 150 µs after the FRAP experiment ends.

Published: March 2010
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camerasBiophotonicscamera-based imagingEuropefluorescence loss in photobleachingfluorescence recovery after photobleachingfluorescent speckle microscopyFRAPGermanyImagingMicroscopymultipoint bleachingOlympus cell^FRAP systemOlympus Europa Holdingpattern bleachingphotoactivationphotocontrolphotoconversionphotomanipulationProductsreal-time controllersingle-point bleaching

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